PathSpecific™ Cathepsin G Activity Assay Kit, Colorimetric (CAT#: ZL-1122-WR17)
| Overview | |
|---|---|
| Description | The kit can be used to quantitatively measure the cathepsin G activity in cell lysate, serum, and tissue lysate. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Cathepsin G protease in biological samples. |
| Application Notes | The kit provides a highly sensitive and accurate assay for quantifying the activity of Cathepsin K in biological samples. |
| Molecular Mechanism of Action | The assay is based on the cleavage of a synthetic substrate by Cathepsin G, resulting in the release of a dye group known as p-NA (4-Nitroaniline). The presence of Cathepsin G can be detected by measuring the absorbance of the p-NA at 405 nm. In the presence of a Cathepsin G specific inhibitor, the cleavage of the substrate and the release of p-NA will be halted. |
| Assay Measures | Colorimetric-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including assay buffer, cathepsin G inhibitor, p-NA standard (0.1 M), positive control, and substrate. |
| Target | Cathepsin G |
| Species Reactivity | Mammals, Other species |
| Format | Supplied with colorimetric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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