PathSpecific™ Cathepsin H Activity Assay Kit, Fluorometric (CAT#: ZL-1122-WR18)
| Overview | |
|---|---|
| Description | The kit is used for the quantitative measurement of cathepsin H activity in cell lysate and tissue lysate. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Cathepsin H protease in biological samples. |
| Application Notes | The kit is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin) |
| Molecular Mechanism of Action | The assay is based on the detection of cathepsin-H activity through the cleavage of a specific substrate, resulting in the release of a fluorescent product. The released fluorescence can be measured using a fluorometer or fluorescence plate reader. This assay is optimized for high sensitivity and is suitable for use in a 96-well plate format. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including CH cell lysis buffer, CH inhibitor (1 mM), CH reaction buffer, and CH substrate R-AFC (10 mM). |
| Target | Cathepsin H |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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