PathSpecific™ Cathepsin K Activity Assay Kit, Fluorometric (CAT#: ZL-1122-WR19)
| Overview | |
|---|---|
| Description | The kit is utilized to measure the cathepsin K activity in cell and tissue lysates quantitatively. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Cathepsin K protease in biological samples. |
| Application Notes | The kit is a fluorescence-based assay that utilizes a specific substrate sequence for cathepsin K, allowing for the quantitative measurement of cathepsin K activity in biological samples. |
| Molecular Mechanism of Action | The assay is based on the ability of cathepsin K to cleave the synthetic substrate RF-AFC, resulting in the release of fluorescent AFC. The released AFC can be quantified using a fluorometer or fluorescence microplate reader, allowing for the measurement of cathepsin K activity. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including CK cell lysis buffer, CK inhibitor (1mM), CK reaction buffer, and CK substrate Ac-LR-AFC (10mM). |
| Target | Cathepsin K |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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