PathSpecific™ Cathepsin L Activity Assay Kit, Fluorometric (CAT#: ZL-1122-WR20)
| Overview | |
|---|---|
| Description | The kit can be applied to measure the cathepsin L activity in cell lysate, plasma, serum, and tissue lysate on a quantitative level. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Cathepsin L protease in biological samples. |
| Application Notes | The kit is an assay based on fluorescence that uses the preferred cathepsin-L substrate sequence, FR, tagged with AFC (amino-4-trifluoromethyl coumarin). |
| Molecular Mechanism of Action | The assay is based on the cleavage of the synthetic substrate by cathepsin L, releasing the fluorogenic group AFC. The released AFC can be quantified using a spectrophotometer at Em = 505 nm, providing a direct measurement of cathepsin L activity in the sample. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including CL buffer, CL inhibitor (1 mM), CL positive control (human), CL substrate Ac-FR-AFC (10 mM), and DTT. |
| Target | Cathepsin L |
| Species Reactivity | Mammals, Other species |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
Online Inquiry