PathSpecific™ Neutrophil Elastase Activity Assay Kit, Fluorometric (CAT#: ZL-1122-WR23)
| Overview | |
|---|---|
| Description | The kit is used to quantitatively assess Elastase activity in cell culture supernatants, serum, and plasma samples. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Elastase protease in biological samples. |
| Application Notes | This kit is designed for a convenient, quick detection of as little as 1 ng of Neutrophil Elastase in a diverse range of samples, lending itself to applications in enzyme kinetics study, drug discovery, and high throughput screening (HTS). |
| Molecular Mechanism of Action | The kit is designed to measure the activity of neutrophil elastase (NE) by monitoring the cleavage of a synthetic substrate and the subsequent release of a fluorophore, AFC, which can be detected using a fluorescence microplate reader at Ex/Em 380/500 nm. |
| Assay Measures | Fluorogenic-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 100 tests |
| Components | The kit is supplied with an assay kit including NE assay buffer, NE dilution buffer, NE enzyme standard, and NE substrate. |
| Target | Elastase |
| Species Reactivity | Mammals, Other species |
| Sensitivity | 1 ng |
| Format | Supplied with fluorometric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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