PathSpecific™ Factor VIIa Human Chromogenic Activity Assay Kit, Colorimetric (CAT#: ZL-1122-WR25)
| Overview | |
|---|---|
| Description | The kit is used to quantitatively measure the Factor VIIa activity in cell culture supernatant and plasma. |
| Features | Ready-used for enzyme activity assay. |
| Applications | Kit is used to study the Factor VIIa protease in biological samples. |
| Application Notes | The assay combines immunofunctional and indirect amidolytic techniques to assess the efficacy of Factor VIIa in activating Factor X (FX) to Factor Xa. This application is useful for studying the mechanisms of coagulation and evaluating the effectiveness of potential therapeutics targeting this pathway. |
| Molecular Mechanism of Action | The kit measures the activity of Factor VIIa by quantifying the amount of FXa generated using a specific substrate. The reaction releases a yellow chromophore, which can be measured by the change in absorbance at 405 nm. |
| Assay Measures | Colorimetric-based detection |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with an assay kit including 10X diluent M concentrate, 20X wash buffer concentrate, assay diluent, Factor Xa substrate, human Factor VIIa microplate, human Factor VIIa standard, human Factor X, and sealing tapes. |
| Target | Factor VIIa |
| Species Reactivity | Human |
| Sensitivity | 0.007 µg/ml |
| Format | Supplied with colorimetric assay kit. |
| Storage | Store at -20°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
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