PathSpecific™ Mouse/Human/Rat FAK (Phospho-Tyr407) Cell-Based Phosphorylation ELISA Kit (CAT#: ZL-0524-WR51)

Overview
DescriptionThis kit is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human FAK / Focal Adhesion Kinase in samples of Adherent Cell Cultures.
FeaturesReady-used for phosphorylation assay.
ApplicationsUseful for measuring the relative amounts of phosphorylated FAK in cultured cells as well as screening for the effects that various treatments, inhibitors, or activators have on FAK phosphorylation.
Assay MeasuresColorimetric
Research AreasKinase Drug Discovery; Signal Pathway;
Specification
Size1 EA
ComponentsThis kit includes User Manual, 96-well Cell Culture Microplate, Blocking Buffer, Detection Antibodies, HRP-Conjugate, Substrate, Stop Solution, Crystal Violet Solution, SDS Solution, and Adhesive Plate Sealers.
TargetFAK
Species ReactivityMouse, Human, Rat
Target/DescriptionQualitative assessment of FAK levels is conducted using an indirect ELISA method. Here, specific primary antibodies bind to the FAK, and HRP-conjugated secondary antibodies bind to the primary antibodies. This interaction enables the HRP enzyme of the secondary antibodies to catalyze a colorimetric reaction when a substrate is added.
FormatColorimetric Assay Kit
StorageStore at 4°C.
HandlingPlease handle the product at appropriate storage condition once received.
ShippingIce bags
Regulatory StatusRUO

For Research Use Only.

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