PathSpecific™ HDAC2 Assay Kit (Fluorogenic) (CAT#: ZL-0224-WR41)
| Overview | |
|---|---|
| Description | The kit is a complete assay system for the detection and quantification of the HDAC2 activity in samples, suitable for screening and analysis purposes. |
| Features | Ready-used for enzymatic activity assay |
| Applications | Kit is used for compound screening and profiling. |
| Application Notes | Excellent for exploring the kinetics of enzymes, screening small molecule inhibitors, and HTS applications. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with HDAC2, fluorogenic HDAC substrate 3, HDAC developer (contains trichostatin A), trichostatin A (200 µM) in DMSO, HDAC assay buffer, and low-binding NUNC black microtiter plate. |
| Target | HDAC2 |
| Target/Description | The HDAC2 assay kit utilizes a fluorogenic substrate and developer combo for streamlined analysis of HDAC activity. This kit simplifies the process by eliminating the need for radioactivity, extraction, and chromatography steps typically found in conventional assays. Administering just two straightforward steps on a microplate, users can evaluate the HDAC2 activity level efficiently. Initially, the acetylated lysine-containing substrate is incubated with purified HDAC2. The ensuing deacetylation primes the substrate for treatment with the Lysine Developer, generating a fluorescent signal that can be quantified using a fluorescence reader. |
| Format | Supplied with a Fluorogenic assay kit |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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