PathSpecific™ HDAC5 Assay Kit (Fluorogenic) (CAT#: ZL-0224-WR44)
| Overview | |
|---|---|
| Description | The kit is a complete assay system for the detection and quantification of the HDAC5 activity in samples, suitable for screening and analysis purposes. |
| Features | Ready-used for enzymatic activity assay |
| Applications | Kit is used for compound screening and profiling. |
| Application Notes | Excellent for exploring the kinetics of enzymes, screening small molecule inhibitors, and HTS applications. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with HDAC5 human recombinant enzyme, fluorogenic HDAC substrate class 2A, HDAC developer (contains trichostatin A), trichostatin A (1 mM) in DMSO, HDAC assay buffer, and low binding NUNC black microtiter plate. |
| Target | HDAC5 |
| Target/Description | The HDAC5 assay kit simplifies HDAC5 activity analysis on a microtiter plate, utilizing a unique fluorogenic substrate and developer. Following incubation with purified HDAC5, the deacetylation process sensitizes the substrate for treatment with the Lysine Developer, generating a measurable fluorophore output that can be quantified using a fluorescence reader. This method eliminates the need for radioactivity, extraction, or chromatography. |
| Format | Supplied with a Fluorogenic assay kit |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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