PathSpecific™ HDAC7 Assay Kit (Fluorogenic) (CAT#: ZL-0224-WR46)
| Overview | |
|---|---|
| Description | The kit is a complete assay system for the detection and quantification of the HDAC7 activity in samples, suitable for screening and analysis purposes. |
| Features | Ready-used for enzymatic activity assay |
| Applications | Kit is used for compound screening and profiling. |
| Application Notes | Excellent for exploring the kinetics of enzymes, screening small molecule inhibitors, and HTS applications. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with HDAC7 human recombinant enzyme, fluorogenic HDAC substrate class 2A, HDAC developer, trichostatin A (1 mM) in DMSO, HDAC assay buffer, and low binding NUNC black microtiter plate. |
| Target | HDAC7 |
| Target/Description | The HDAC7 assay kit offers a convenient and efficient way to assess HDAC7 activity levels. By utilizing a unique fluorogenic substrate and developer combination, this kit eliminates the need to handle radioactivity, extraction, and chromatography involved in traditional assays. With just two simple steps on a microtiter plate, users can analyze the level of HDAC7 activity. The process involves incubating the HDAC fluorometric substrate with purified HDAC7, which deacetylates the substrate. Subsequent treatment with the Lysine Developer generates a fluorophore that can be quantified using a fluorescence reader. |
| Format | Supplied with a Fluorogenic assay kit |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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