PathSpecific™ HDAC8 Assay Kit (Fluorogenic) (CAT#: ZL-0224-WR47)
| Overview | |
|---|---|
| Description | The kit is a complete assay system for the detection and quantification of the HDAC8 activity in samples, suitable for screening and analysis purposes. |
| Features | Ready-used for enzymatic activity assay |
| Applications | Kit is used for compound screening and profiling. |
| Application Notes | Excellent for exploring the kinetics of enzymes, screening small molecule inhibitors, and HTS applications. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with HDAC8 human recombinant enzyme, fluorogenic HDAC substrate class 2A, HDAC developer, trichostatin A (1 mM) in DMSO, HDAC assay buffer, and low binding NUNC black microtiter plate. |
| Target | HDAC8 |
| Target/Description | The HDAC 8 assay kit simplifies the process of analyzing HDAC 8 activity levels through the use of a unique fluorogenic substrate and developer. Unlike traditional assays, utilizing this kit eliminates the need for radioactivity, extraction, and chromatography procedures. The testing procedure involves two steps conducted on a microtiter plate, initially incubating the HDAC fluorometric substrate with the HDAC8 enzyme and then treating the sensitized substrate with a Lysine Developer to produce a measurable fluorophore. |
| Format | Supplied with a Fluorogenic assay kit |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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