PathSpecific™ IDH1 (R132H) Isocitrate Dehydrogenase Assay Kit-Fluorogenic (CAT#: ZL-0424-WR33)
| Overview | |
|---|---|
| Description | This kit is designed to measure IDH1(R132H) activity for screening and profiling applications by measuring NADPH consumption. |
| Features | Ready-used for isocitrate dehydrogenase assay. |
| Applications | Useful for screening small molecular inhibitors for drug discovery and HTS applications. |
| Assay Measures | Fluorometric |
| Research Areas | Kinase Drug Discovery; Signal Pathway |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | This kit includes enough purified recombinant IDH1(R132H) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions. |
| Target | IDH1(R132H) |
| Target/Description | Enzymes known as isocitrate dehydrogenases facilitate the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations in isocitrate dehydrogenase 1 and 2 (IDH1 and IDH2) have been observed in various forms of human cancer. In contrast to the wild-type IDH1, which generates α-ketoglutarate (α-KG) by reducing NADP⁺ to NADPH, the IDH1(R132H) mutant facilitates the conversion of α-ketoglutarate to 2-hydroxyglutarate (2-HG) by oxidizing NADPH to NADP⁺. |
| Format | Fluorometric assay kit. |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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