PathSpecific™ IDH2 (R140Q) Isocitrate Dehydrogenase Assay Kit-Fluorogenic (CAT#: ZL-0424-WR34)
| Overview | |
|---|---|
| Description | This kit is designed to measure IDH2(R140Q) activity for screening and profiling applications by measuring NADPH consumption. |
| Features | Ready-used for isocitrate dehydrogenase assay. |
| Applications | Useful for screening small molecular inhibitors for drug discovery and HTS applications. |
| Assay Measures | Fluorometric |
| Research Areas | Kinase Drug Discovery; Signal Pathway |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | This kit includes enough purified recombinant IDH2(R140Q) enzyme, α-KG, NADPH, NADPH detection reagents, and assay buffer for 100 enzyme reactions. |
| Target | IDH2 (R140Q) |
| Target/Description | Isocitrate dehydrogenases are crucial enzymes that play a key role in cellular metabolism by converting isocitrate to α-ketoglutarate. However, mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been linked to the development of various types of cancer. Specifically, the IDH2(R140Q) mutation leads to the production of 2-hydroxy-glutarate (2-HG) instead of α-ketoglutarate, which can contribute to tumorigenesis. This mutation has been identified in several cancer types, including glioma, chondrosarcoma, acute myeloid leukemia, as well as a small percentage of prostate, lung, and colon cancers. |
| Format | Fluorometric assay kit. |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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