PathSpecific™ Human MLL3 Complex Assay Kit, Chemiluminescent (CAT#: ZL-0224-WR10)
| Overview | |
|---|---|
| Description | The kit provides a reliable method for quantifying MLL3 activity in various biological samples, utilizing purified MLL3 and associated complex components (WDR5, RbBP5, Ash2, and DPY30) for accurate screening and profiling. |
| Features | Ready-used for enzymatic activity assay |
| Applications | Kit is used for compound screening and profiling. |
| Application Notes | Excellent for exploring the kinetics of enzymes and HTS applications. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with 8-well strips pre-coated with histone H3 peptide substrate, an antibody against methylated lysine on Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and enough purified MLL3 enzyme complex for 100 enzyme reactions. |
| Target | MLL3 Complex |
| Target/Description | The assay begins by adding MLL3 enzyme complex and S-adenosylmethionine to a pre-coated plate with a histone H3 peptide substrate, incubated with or without a test compound. A primary antibody is then applied, followed by an HRP-labeled secondary antibody. Ultimately, the HRP substrate is added to generate chemiluminescence, which is measured using a chemiluminescence reader. The signal reflects the enzymatic activity of the MLL3/WARD complex. The kit's user-friendly format allows for quick and easy detection of methyltransferase activity in just three simple steps on a microtiter plate. |
| Format | Supplied with a chemiluminescent assay kit |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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