PathSpecific™ PDE4D Phosphodiesterase Assay Kit-Luminescent (CAT#: ZL-0424-WR46)
| Overview | |
|---|---|
| Description | This kit is designed for screening inhibitors of PDE4D7 in cultured cells. |
| Features | Ready-used for phosphodiesterase assay. |
| Applications | Screen PDE4D inhibitors for drug discovery & Monitor cAMP/PDE4D signaling pathway activity. |
| Assay Measures | Luminescent |
| Research Areas | Kinase Drug Discovery; Signal Pathway |
| Specification | |
|---|---|
| Size | 500 tests |
| Components | This kit includes CRE luciferase reporter (premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency) & a PDE4D7 expression vector. |
| Target | PDE4D |
| Target/Description | The assay involves transfecting cells with a CRE luciferase reporter. CRE reporter contains the firefly luciferase gene controlled by cAMP response element (CRE). Elevation of cAMP activates CREB to bind CRE, leading to luciferase expression. Forskolin boosts cAMP levels, inducing luciferase expression. Co-transfection of PDE4D7 reduces forskolin-induced cAMP and lowers luciferase levels. Treatment with a PDE4D inhibitor restores cAMP levels and increases luciferase activity. |
| Format | Luminescent assay kit |
| Storage | Store at -20°C. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Ice bags |
| Regulatory Status | RUO |
For Research Use Only.
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