PathSpecific™ PDE7A Phosphodiesterase Assay Kit-Fluorescence Polarization (CAT#: ZL-0424-WR47)
| Overview | |
|---|---|
| Description | This kit is designed for identification of PDE7A inhibitors using fluorescence polarization. |
| Features | Ready-used for phosphodiesterase assay. |
| Applications | Useful for examining enzyme kinetics and screening small molecule inhibitors for drug discovery in high throughput screening (HTS) applications. |
| Assay Measures | Fluorescence Polarization |
| Research Areas | Kinase Drug Discovery; Signal Pathway |
| Specification | |
|---|---|
| Size | 384 tests |
| Components | This kit includes purified PDE7A enzyme, fluorescently labeled PDE7A substrate (cAMP), binding agent, and PDE assay buffer for 384 enzyme reactions. |
| Target | PDE7A |
| Target/Description | The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE7A to the binding agent. PDE7A catalyzes the hydrolysis of the phosphodiester bond in dye-labeled cyclic adenosine monophosphate (cAMP). Nanoparticle beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cAMP. Since the degree of polarization of a fluorophore is inversely related to its molecular rotation, dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light. Conversely, dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. change to another description. |
| Format | Fluorescence polarization assay kit. |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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