PathSpecific™ Human Serine Threonine-protein Phosphatase PP1-alpha Catalytic Subunit, PP1A ELISA Kit (CAT#: ZL-1122-WR44)
| Overview | |
|---|---|
| Description | The kit is an ELISA kit designed for accurate measurement of the levels of human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PP1A) in serum, plasma, cell culture supernatants, or other biological samples. |
| Features | Ready-used for quantitative measurement. |
| Applications | Kit is used to study the PP1A phosphatase in serum, plasma, cell culture supernatants, or other biological fluids. |
| Application Notes | This sandwich kit is designed for the precise quantification of Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (also referred to as PPP1CA) in various biological samples, including serum, plasma, cell culture supernatants, Ascites, tissue homogenates, or other biological fluids. |
| Molecular Mechanism of Action | After being introduced to the sample, PP1A binds to the antibodies coated on the wells. Next, biotinylated Human PP1A Antibody is introduced to the sample, where it binds to PP1A. Subsequently, the biotinylated PP1A antibody binds to streptavidin-HRP. During a washing step following incubation, unbound streptavidin-HRP is removed. Following the addition of the substrate solution, color changes in accordance with the concentration of human PP1A. At 450 nm, absorbance is measured after the addition of an acidic stop solution to halt the process. |
| Assay Measures | ELISA method |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 48 tests; 96 tests |
| Components | The kit is supplied with an assay kit including pre-coated ELISA plate, standard solution, standard diluent, streptavidin-HRP, stop solution, substrate solution A & B, wash buffer concentrate (25x), and biotinylated antibody. |
| Target | PP1A |
| Species Reactivity | Human |
| Sensitivity | 6.95ng/L |
| Format | Supplied with ELISA assay kit. |
| Storage | Store at 2-8°C |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Ice packs |
| Regulatory Status | RUO |
For Research Use Only.
Online Inquiry