PathSpecific™ SARS-CoV-2 Mpro Assay Kit (CAT#: ZL-0124-WR46)

Overview
DescriptionThis kit is a quick and user-friendly FRET-based assay that involves only two simple steps. First, the Mpro enzyme is combined with the compound and left to incubate for 30 minutes. Then, the substrate solution is added to initiate the reaction. The fluorescent intensity is easily determined using a fluorescent plate reader at specific excitation and emission wavelengths.
FeaturesReady-used for inhibitors screening assay.
ApplicationsKit is used to screen the Mpro Inhibitors.
Application NotesUseful for screening of Mpro inhibitors.
Research AreasKinase Drug Discovery; Signal Pathway;
Specification
Size1 EA
ComponentsThe kit is supplied with an assay kit including 2X Protease Assay Buffer, 0.5 M DTT, 5 mM Substrate, Recombinant SARS-CoV-2 Mpro, and Black low binding 96 well plate.
TargetSARS-CoV-2 Mpro
Target/DescriptionMpro of Severe acute respiratory Coronavirus 2 (SARS CoV-2 Mpro, also known as SARS-CoV-2 Main protease or SARS-CoV-2 3CL protease) is crucial for viral replication as it processes the polyproteins 1ab at 11 specific cleavage sites. Inhibiting the activity of this enzyme holds promise for developing effective anti-coronaviral therapeutic agents.
FormatSupplied with a FRET-based assay kit.
StorageStore at the appropriate temperature as directed.
HandlingPlease store the kit according to the storage instructions in the kit's manual upon receipt.
ShippingDry ice
Regulatory StatusRUO

For Research Use Only.

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