| Overview | |
|---|---|
| Description | The kit is specifically designed for the detection and quantification of SET1B enzyme activity in biological samples for screening and profiling applications. |
| Features | Ready-used for enzymatic activity assay |
| Applications | Kit is used for compound screening and profiling. |
| Application Notes | Excellent for exploring the kinetics of enzymes, screening small molecule inhibitors, and HTS applications. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with a 96-well plate precoated with histone H3 peptide substrate, an antibody against the methylated lysine 9 residue of Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SET1B enzyme (amino acids 510-1290) for 96 enzyme reactions. |
| Target | SET1B |
| Target/Description | The SetDB1 Assay kit uses a highly precise antibody to detect the methylated K9 residue of Histone H3. Using a simple three step process on a microtiter plate, the kit aids in the detection of methyltransferase activity. Initial incubation with S-adenosylmethionine and the enzyme is followed by introduction of the primary antibody and subsequent treatment with an HRP-tagged secondary antibody. The ensuing chemiluminescence, which can be measured using a reader, indicates the presence of the enzyme. |
| Format | Supplied with a chemiluminescent assay kit |
| Storage | Store at the appropriate temparature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
