| Overview | |
|---|---|
| Description | This kit provides a quantitative in vitro enzyme-linked immunosorbent assay (ELISA) method for detecting the activity of Set8 in various biological fluids, tissue homogenates, and cell lysates for analytical profiling and screening applications. |
| Features | Ready-used for enzymatic activity assay |
| Applications | Kit is used for compound screening and profiling. |
| Application Notes | Excellent for exploring the kinetics of enzymes, screening small molecule inhibitors, and HTS applications. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with enough recombinant purified SET8 enzyme (amino acids 195-252), substrate, antibodies, and all the reagents necessary for detection. |
| Target | SET8 |
| Target/Description | SET8, also known as KMT5A, is a member of the SET domain-containing protein family. This protein specifically monomethylates H4K20me1 and also targets proteins such as p53 and PCNA. SET8 takes part in cell cycle regulation and DNA replication and repair mechanisms. Irregular expression levels of SET8 have been linked to cancer, and particularly high levels are evidenced in some such as breast cancer. Inhibition of SET8 has been seen to enhance cancer cell sensitivity to radiotherapy, making it of interest in creating targeted treatments. |
| Format | Supplied with a chemiluminescent assay kit |
| Storage | Store at the appropriate temparature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
