PathSpecific™ Human SETD2 Assay Kit, Chemiluminescent (CAT#: ZL-0224-WR24)
| Overview | |
|---|---|
| Description | This ELISA kit is utilized to quantitatively measure the activity of SETD2 in various biological samples for screening and profiling applications. |
| Features | Ready-used for enzymatic activity assay |
| Applications | Kit is used for compound screening and profiling. |
| Application Notes | Excellent for exploring the kinetics of enzymes and HTS applications. |
| Research Areas | Kinase Drug Discovery; Signal Pathway; |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | The kit is supplied with a 96-well plate precoated with histone H3 peptide substrate, purified SETD2 and reagents for 100 enzyme reactions. |
| Target | SETD2 |
| Target/Description | The SETD2 assay kit works in a simple three-step process for detecting methyltransferase activity. This kit includes a highly specific antibody recognizing the methylated K36 residue of Histone H3. The first step involves incubating S-adenosylmethionine with a sample containing both assay buffer and methyltransferase enzyme. Afterwards, the primary antibody is added and finally, the plate is treated with HRP-labeled secondary antibody and an HRP substrate. This results in chemiluminescence that can be collected and measured using a chemiluminescence reader. |
| Format | Supplied with a chemiluminescent assay kit |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
Online Inquiry