PathSpecific™ UCHL3 Deubiquitination Assay Kit-Fluorogenic (CAT#: ZL-0424-WR52)
| Overview | |
|---|---|
| Description | This kit is a 96-well format fluorogenic assay designed to measure the activity of the deubiquitinating (DUB) enzyme UCHL3 for screening and profiling applications. |
| Features | Ready-used for deubiquitination assay |
| Applications | Useful for examining enzyme kinetics and screening small molecule inhibitors for drug discovery in high throughput screening (HTS) applications. |
| Assay Measures | Fluorogenic |
| Research Areas | Kinase Drug Discovery; Signal Pathway |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | This kit includes enough purified UCHL3 protein, Ubiquitinated-AMC substrate and assay buffer for 100 reactions. |
| Target | UCHL3 |
| Target/Description | Analyzing the effect of an inhibitor on UCHL3 activity involves preincubating the enzyme with or without the inhibitor before introducing the Ub-AMC substrate. The assay's validation included the use of UbAldehyde, a potent inhibitor targeting DUB subfamilies such as Ubiquitin C-terminal Hydrolases (UCHs), Ubiquitin-Specific Proteases (USPs), Ovarian Tumor Proteases (OTU), and Machado-Josephin Domain (MJD) proteases. |
| Format | Fluorometric assay kit. |
| Storage | Store at -80°C. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
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