PBMC Cytotoxicity Bioassay Kit (CFSE, 7-ADD) (CAT#: ZL-0124-WR45)
| Overview | |
|---|---|
| Description | The kit is designed to assess the cytotoxic potential of PBMC (Peripheral Blood Mononuclear Cells) towards target cells of interest. By utilizing fluorescent markers such as CFSE and 7-AAD, the kit enables the detection of cell cytotoxicity at the individual cell level using flow cytometry. |
| Features | Ready-used for cytotoxicity assay. |
| Applications | Kit is used for cytotoxicity profiling. |
| Application Notes | Examine cellular responses in cytotoxicity assays using flow cytometry analysis. Ideal for evaluating effects of immune engager molecules and candidate antibodies. |
| Research Areas | CAR T-Cell Therapy |
| Specification | |
|---|---|
| Size | 1 EA |
| Components | The kit is supplied with an assay kit including PBMCs, Thaw Medium, and Assay Diluent. |
| Target | PBMCs |
| Target/Description | Lymphocyte-mediated cytotoxicity targets pathogens within cells through cellular immunity. This can be monitored by pre-labelling target cells with CSFE, a dye that permeates cell membranes and stains live cells. After incubation with these cells, 7-AAD, a dye that intercalates with DNA, is added to identify dying cells. |
| Format | Supplied with a bioassay kit |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please store the kit according to the storage instructions in the kit's manual upon receipt. |
| Shipping | Dry ice |
| Regulatory Status | RUO |
For Research Use Only.
Online Inquiry