Transcription Activator-like Effector Nuclease Service

With advanced technology and rich experience, Creative Biolabs is providing the Transcription Activator-like Effector Nuclease Service for our global clients, especially those who are currently working on drug target validation. The nucleases used in our platform provide high specificity and optimized digestion conditions possible. We help avoid the pitfalls of variability in assays and benefit from the efficiency and accuracy of a dedicated facility.

In the pharmacological industry, tons of drugs are blocked because of their undesired performances in the clinical trials when a great deal of money has been invested. Therefore, drug discovery stands out as a quite important and essential way for reducing the cost and risk. Drug discovery is the process by which drugs are discovered and designed. Loss-of-function mutation is a great tactic for discovering drug. This is definitely one of the best ways to be completely certain that a protein plays a key role in a given disease is to make that protein deficient and see if the cell or the animal model is resistant to the disease when the drug is added.

The transcription activator-like effector (TALE) nuclease cleaves specific DNA target nucleotide. It is composed of two modules, the TALE and the nuclease. Found in plant virulence factors from Xanthomonas bacteria, the transcription activator-like effector (TALE) was reported to have DNA binding ability. The DNA binding domain contains a repeated highly conserved 33-34 amino acid sequence with the exception at the 12^th and 13^th amino acids referred to as repeat variable di-residue (RVD). These two positions are called the Repeat Variable Di-residue (RVD), which are highly variable and determine the correlation with specific nucleotide recognition. The nuclease is an enzyme that cleaves the phosphodiester bonds between monomers of nucleic acids. In living cells, nucleases variously generate single- or double-stranded breaks on their targets. The nuclease we use in our platform is FokI, a bacterial type IIS restriction endonuclease. TALEN uses TALE for recognizing DNA while utilizes FokI catalytic domain as the DNA cleavage domain. By taking advantage of the fact that endogenous DNA repair machinery employs non-homologous end joining (NHEJ) pathway and homology-directed repair (HDR) pathway, these reagents can be used to precisely alter the genomes of higher organisms.

Fig.1 Schematic Representation of Transcription Activator-like Effector (TALE) Nuclease (Joung, 2013).

Application:

• TALEN has been widely utilized to generate NHEJ-mediated mutations
• Drug discovery and target validation
• Introducing insertion using double-stranded templates (HDR events)
• TALEN is not limited to generating mutagenesis in mouse embryonic stem
• Potential therapeutic approach for genetic-based diseases

Service note:
Our clients are supposed to provide target gene name, sequence or GeneBank ID.
Creative Biolabs will offer:

• RVD module plasmid
• TALEN activity report
• PCR-based detection report
• Single colony

Creative Biolabs has accumulated extensive experience to offer gene editing services. Our experienced scientists will assist you in designing the research program that best suits your purpose and advances your research.If you are interested in using our Transcription Activator-like Effector Nuclease service to support your target validation and identification program, please feel free to contact us for more details.

Reference

1. Joung, J.K. and J.D. Sander. (2013). “TALENs: a widely applicable technology for targeted genome editing”. Nat Rev Mol Cell Biol 14(1), 49-55.
   

For Research Use Only.