Hi-Affi™ 96-Well Comet Assay Kit-Fluorometric (CAT#: ZL-0424-WR8)
| Overview | |
|---|---|
| Description | This kit applies a single-cell gel electrophoresis test (SCGE) to assess cellular DNA damage in a straightforward manner. |
| Features | Ready-used for DNA damage assay. |
| Applications | Efficient for the measurement of cellular DNA damage. |
| Assay Measures | Fluorescence Microscopy |
| Research Areas | Oxidative stress; Cell Damage; Drug Discovery |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | This kit includes a 96-Well Comet Slide, Comet Agarose, Vista Green DNA Dye, EDTA Solution, and Lysis Solution. |
| Target/Description | Initially, cells are combined with molten agarose before being processed with a lysis buffer and alkaline solution to relax and denature the DNA. The samples are then electrophoresed in a horizontal chamber to distinguish between intact DNA and damaged fragments. Subsequently, the samples are dried, stained with a DNA dye, and observed using epifluorescence microscopy. Damaged DNA, which includes cleavage and strand breaks, migrates further than intact DNA, forming a distinctive "comet tail" shape. |
| Format | Fluorometric assay kit. |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Ice bags |
| Regulatory Status | RUO |
For Research Use Only.
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