| Overview | |
|---|---|
| Description | Creative Biolabs offers a wide range of dye reagents and kits suitable for evaluating cell biological activity such as apoptosis, endocytosis and can be used in a number of areas: drug discovery, toxicity testing and immunology. Apoptosis Detection Kit, Annexin V-AF 488 & PI is used for identification and enumeration of dead cells, such as apoptotic or necrotic cells, by FC, IF. |
| Features | Convenient, and simple steps; Be compatible with various antibody function study platforms; Reduced signal variability and improved timing in sensitive experiments; |
| Applications | Be used for the identification and enumeration of dead cells, such as apoptotic or necrotic cells, by flow cytometry. |
| Molecular Mechanism of Action | Kit has been developed for detection and discrimination of apoptotic, necrotic, and dead cells. In principle, apoptotic cells are stained positively for Annexin V conjugation that binds to phosphotidylserine (PS), but are negative for staining with propidium iodide (PI). Dead cells are stained positive for Annexin V conjugation and PI, whereas viable cells are negative for both Annexin V conjugation and PI. |
| Assay Measures | Fluorescence |
| Research Areas | Drug Discovery and Toxicity Testing; Immunology |
| Specification | |
|---|---|
| Size | 1 kit |
| Components | Annexin V - AF488; Annexin Binding solution; Propidium Iodide (PI) solution; |
| Storage | Store protected from light at 2-8 °C. Do not freeze. |
| Handling | Please place the product at proper storage condition once received. |
| Shipping | Ice packs |
For Research Use Only.
