| Overview | |
|---|---|
| Description | Creative Biolabs offers a wide range of dye reagents and kits suitable for evaluating cell biological activity such as apoptosis, endocytosis and can be used in a number of areas: drug discovery, toxicity testing and immunology. Apoptosis Detection Kit, Annexin V-APC & 7-AAD is used for identification and enumeration of dead cells, such as apoptotic or necrotic cells, by FC, IF. |
| Features | Convenient, and simple steps; Be compatible with various antibody function study platforms; Reduced signal variability and improved timing in sensitive experiments; |
| Applications | Be used for the identification and enumeration of dead cells, such as apoptotic or necrotic cells, by flow cytometry. |
| Molecular Mechanism of Action | Kit has been developed for detection and discrimination of apoptotic, necrotic, and dead cells. In principle, apoptotic cells are stained positively for Annexin V conjugation that binds to phosphotidylserine (PS). As cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently the 7-AAD viability dye passes into the cell. Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and 7-AAD. |
| Assay Measures | Fluorescence |
| Research Areas | Drug Discovery and Toxicity Testing; Immunology |
| Specification | |
|---|---|
| Size | 1 kit |
| Components | Annexin V, 1X 100 Tests; Annexin V Binding Buffer, 1 x 50 Ml; 7-AAD, 1X 200 Tests; |
| Storage | Store protected from light at 2-8 °C. Do not freeze. |
| Handling | Please place the product at proper storage condition once received. |
| Shipping | Ice packs |
For Research Use Only.
