Apoptosis Detection Kit, Annexin V-APC & 7-AAD (CAT#: DR-LX017)

Name: Apoptosis Detection Kit, Annexin V-APC & 7-AAD
SKU: DR-LX017
Rating: 5 (1 reviews)

Overview
Description	Creative Biolabs offers a wide range of dye reagents and kits suitable for evaluating cell biological activity such as apoptosis, endocytosis and can be used in a number of areas: drug discovery, toxicity testing and immunology. Apoptosis Detection Kit, Annexin V-APC & 7-AAD is used for identification and enumeration of dead cells, such as apoptotic or necrotic cells, by FC, IF.
Features	Convenient, and simple steps; Be compatible with various antibody function study platforms; Reduced signal variability and improved timing in sensitive experiments;
Applications	Be used for the identification and enumeration of dead cells, such as apoptotic or necrotic cells, by flow cytometry.
Molecular Mechanism of Action	Kit has been developed for detection and discrimination of apoptotic, necrotic, and dead cells. In principle, apoptotic cells are stained positively for Annexin V conjugation that binds to phosphotidylserine (PS). As cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently the 7-AAD viability dye passes into the cell. Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and 7-AAD.
Assay Measures	Fluorescence
Research Areas	Drug Discovery and Toxicity Testing; Immunology

Specification
Size	1 kit
Components	Annexin V, 1X 100 Tests; Annexin V Binding Buffer, 1 x 50 Ml; 7-AAD, 1X 200 Tests;
Storage	Store protected from light at 2-8 °C. Do not freeze.
Handling	Please place the product at proper storage condition once received.
Shipping	Ice packs

For Research Use Only.