PathSpecific™ Glucose Uptake Assay Kit-Fluorimetric (CAT#: ZL-0725-WR22)
| Overview | |
|---|---|
| Description | Creative Biolabs' PathSpecific™ Glucose Uptake Assay Kit-Fluorimetric offers a sensitive and non-radioactive test for assessing the uptake of glucose in cultured cells. |
| Features | Non-radioactive, sensitive |
| Applications | To quantify glucose uptake in cells. |
| Molecular Mechanism of Action | In this assay, 2-deoxyglucose (2-DG) is internalized via glucose transporters and converted to 2-DG-6-phosphate (2-DG6P). The intracellular accumulation of the non-metabolizable 2-DG6P directly correlates with cellular glucose uptake. Subsequently, 2-DG6P is enzymatically oxidized, producing NADPH, which is then quantitatively detected using a fluorogenic NADPH sensor and measured with a fluorescence microplate reader. |
| Assay Measures | Fluorimetric |
| Research Areas | Glucose uptake assay; Drug discovery |
| Excitation | 540 nm |
| Emission | 590 nm |
| Specification | |
|---|---|
| Size | 1 EA |
| Format | 100 Tests;500 Tests |
| Storage | Store at -20 °C, and protect from light. |
| Handling | Please place the product at proper storage condition once received. |
| Shipping | Ice bags |
| Regulatory Status | RUO |
For Research Use Only.
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