Hi-Affi™ UV-Induced DNA Damage ELISA Kit (6-4PP Quantitation)-Colorimetric (CAT#: ZL-0424-WR11)
| Overview | |
|---|---|
| Description | This enzyme immunoassay kit is designed to quickly identify and measure 6-4PP from various DNA samples. |
| Features | Ready-used for DNA damage assay. |
| Applications | Useful for measurement of 6-4PP formation in DNA isolated from cells or tissue. |
| Assay Measures | Colorimetric |
| Research Areas | Oxidative stress; Cell Damage; Drug Discovery |
| Specification | |
|---|---|
| Size | 96 tests |
| Components | This kit includes a DNA High-Binding Plate, DNA Binding Solution, Anti-6-4PP Antibody, HRP Conjugate-Secondary Antibody, Assay Diluent, Wash Buffer, Substrate Solution, Stop Solution, 6-4PP-DNA Standard, and Reduced DNA Standard. |
| Target/Description | Following denaturation, 6-4PP-DNA standards or unknown DNA samples are loaded onto a DNA high-binding plate. The samples are then probed with an anti-6-4PP antibody and a secondary antibody conjugated to HRP. Detection occurs through a colorimetric reaction with a substrate, and the absorbance is quantified using a plate reader. The 6-4PP content in unknown samples is determined by comparing the absorbance values to a standard curve generated from known 6-4PP-DNA standards. |
| Format | Colorimetric assay kit. |
| Storage | Store at the appropriate temperature as directed. |
| Handling | Please handle the product at appropriate storage condition once received. |
| Shipping | Ice bags |
| Regulatory Status | RUO |
For Research Use Only.
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