PathSpecific™ Glucose Uptake Assay Kit-Colorimetric (CAT#: ZL-0725-WR23)

Datasheet
Overview
DescriptionCreative Biolabs' PathSpecific™ Glucose Uptake Assay Kit-Colorimetric offers a sensitive and non-radioactive test for assessing the uptake of glucose in tissue or cultured cells.
ApplicationsTo quantify glucose uptake in tissue or cultured cells.
Molecular Mechanism of ActionIn this assay, 2-deoxyglucose (2-DG) is internalized by glucose transporters and phosphorylated to 2-deoxyglucose-6-phosphate (2-DG6P). This non-metabolizable product then accumulates within the cells, providing a direct measure of glucose uptake. The accumulated 2-DG6P is subsequently oxidized by an enzyme, producing NADPH, which is then quantified using a chromogenic sensor. The resulting signal can be detected by an absorbance microplate reader, by calculating the optical density ratio at 570 nm to 610 nm.
Assay MeasuresColorimetric
Research AreasGlucose uptake assay; Drug discovery
Absorbance570, 610 nm
Specification
Size1 EA
Format100 Tests;500 Tests
StorageStore at -20 °C, and protect from light.
HandlingPlease place the product at proper storage condition once received.
ShippingIce bags
Regulatory StatusRUO

For Research Use Only.

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